Ho tloha qalong ea seoa sena, tlhahlobo ea tlhahlobo ea mafu e phethile karolo ea bohlokoa ho laola ho ata ha SARS-CoV-2, vaerase e bakang.COVID 19.Liteko tse potlakileng tsa antigene etsoang lapeng kapa maemong a kliniki fana ka sephetho ka metsotso e 15 kapa ka tlase ho moo.Hang ha motho a fumanoa a e-na le lefu lena, kapele a ka batla thuso ea bongaka ’me a ikarola ho ba bang.Empa ha mefuta e mecha ea vaerase e hlaha, mefuta eo e kanna ea se bonwe ke liteko tsena.
Liteko tse ngata tse potlakileng tsa antigen li etselitsoe ho bona protheine ea SARS-CoV-2 nucleocapsid kapa N-protein.Protheine ena e fumanoa ka bongata likarolong tsa kokoana-hloko le batho ba tšoaelitsoeng.Setsi sa tlhahlobo se potlakilengs hangata a na le li-antibodies tse peli tse fapaneng tse tlamang likarolong tse fapaneng tsa protheine ea N.Ha lesole la 'mele le tlama ho protheine ea N ka sampole, ho hlaha mola o mebala kapa lets'oao le leng sephuthelong sa tlhahlobo, se bontšang tšoaetso.
Protein N e na le likarolo tse 419 tsa amino acid.E 'ngoe le e' ngoe ea tsona e ka nkeloa sebaka ke amino acid e 'ngoe ka phetoho.Sehlopha sa lipatlisiso se etelletsoeng pele ke Ph.D.Philip Frank le Eric Ortlund ba Univesithi ea Emory ba ile ba ikemisetsa ho batlisisa hore na phetoho ena e le 'ngoe ea amino acid e ama ts'ebetso ea tlhahlobo e potlakileng ea antigen joang.Ba sebelisitse mokhoa o bitsoang deep mutation scanning ho lekola ka nako e le 'ngoe hore na phetoho e' ngoe le e 'ngoe ea protheine ea N ea vaerase e ama joang ho tlama le antibody ea tlhahlobo.Liphetho tsa bona li phatlalalitsoe ho Cell ka la 15 Loetse 2022.
Bafuputsi ba thehile laeborari e felletseng ea liphetoho tsa protheine tse ka bang 8,000 N.Mefuta ena e etsa karolo e fetang 99.5% ea liphetoho tsohle tse ka bang teng.Ba ile ba lekola hore na mofuta o mong le o mong o sebelisana joang le li-antibodies tse 17 tse fapaneng tse sebelisoang litekong tse 11 tse fumanehang khoebong tsa antigen, ho kenyelletsa le tse tloaelehileng.lisebelisoa tsa lapeng.
Sehlopha se hlahlobile hore na ke liphetoho life tsa N-protein tse amang temoho ea li-antibody.Ho ipapisitsoe le tlhaiso-leseling ena, ba thehile "profil ea ho baleha phetoho" bakeng sa antibody e 'ngoe le e' ngoe ea tlhahlobo.Boemo bona bo supa liphetoho tse itseng ho protheine ea N tse ka amang bokhoni ba lesole la 'mele la ho ikamahanya le sepheo sa lona.Tlhahlobo e bonts'itse hore li-antibodies tse sebelisitsoeng litekong tse potlakileng tsa kajeno li lemoha le ho tlama mefuta eohle ea nakong e fetileng le ea hona joale ea SARS-CoV-2 ea ho tšoenyeha le ho tšoenyeha.
Leha li-antibodies tse 'maloa li lemoha sebaka se le seng sa protheine ea N, bafuputsi ba fumane hore lesole le leng le le leng le na le lets'oao le ikhethileng la liphetoho tsa ho phonyoha.Ha vaerase ea SARS-CoV-2 e ntse e tsoela pele ho fetoha le ho hlahisa mefuta e mecha, datha ena e ka sebelisoa ho tšoaea li-antibodies tsa lisebelisoa tsa tlhahlobo tse ka hlokang ho hlahlojoa hape.
"Boitsebiso bo nepahetseng le bo nepahetseng ba batho ba tšoaelitsoeng e ntse e le leano la bohlokoa bakeng sa phokotso ea COVID-19, 'me boithuto ba rona bo fana ka leseli mabapi le liphetoho tsa nako e tlang tsa SARS-CoV-2 tse ka sitisang ho fumanoa," Ortlund o boletse."Liphetho tse boletsoeng mona li re lumella ho ikamahanya le vaerase ena ka potlako ha mefuta e mecha e ntse e tsoela pele ho hlaha, e fana ka litlamorao tsa bophelo bo botle ba bophelo bo botle ba sechaba."
Background: Mutation Deep Scan e lemoha liphetoho tsa ho phonyoha ho SARS-CoV-2 nucleocapsid e sebelisa liteko tsa antigen tse fumanehang hajoale.Frank F., Kin MM, Rao A., Bassit L., Liu H, Bowers HB, Patel AB, Kato ML, Sullivan JA, Greenleaf M., Piantadosi A., Lam VA, Hudson VH, Ortlund EA cell.2022 September 15; 185 (19): 3603-3616.e13.Lekala la Litaba tsa ka Hare: 10.1016/j.cell.2022.08.010.La 29 Phato, 2022 PMID: 36084631.
Lithuso tsa lichelete: Setsi sa Sechaba sa Boitšoaro ba Likokoana-hloko le Bioengineering NIH (NIBIB), Setsi sa Sechaba sa lefu la tsoekere, lefu la tšilo ea lijo le liphio (NIDDK) le National Institute of Allergy and Infectious Diseases (NIAID), American Heart Association.
NIH Research Matters ke ntlafatso ea beke le beke ea liphuputso tsa bohlokoa tsa NIH tse hlahlobiloeng ke litsebi tsa NIH.E hatisitsoe ke Ofisi ea Lipuisano le Litaba tsa Sechaba ea Mookameli oa Mekhatlo ea Sechaba ea Bophelo.